Curriculum vitae                                                              22/06/2007

 

Personal details

Name:                                   LEONARD STOICA

Date and Place of Birth:  11th of November, 1973, Moreni, Romania

Status:                                  Married (two children)

Home address:                  Vormholzstr. 16d, D-44801 Bochum, Germany

Work address:                    Department of Analytical Chemistry, Ruhr-Universität Bochum

Universitätsstr. 150, NC 04/792, D-44780 Bochum, Germany

Email:                                    leonard.stoica@rub.de

Telephone:                          ++49 (0) 234 32-25202

Mobil:                                   ++49 (0)176 66005635

Home:                                  ++49 (0) 234 62-94664

Languages:                         Romanian (native), English (fluent)

Swedish (basic) and German (basic)

 

 

Present position (since May 2005):       Post-Doctoral at Elektroanalytik&Sensorik, Ruhr-University Bochum, Bochum, Germany

    At the moment involved in the following projects:

·         Investigation of local catalytic activity at gas-diffusion electrode (GDE) by means of Scanning Electrochemical Microscopy “ (2007) FKZ:01LS05139, sponsored by Bundesministerium für Bildung und Forschung (BMBF)

·         Local microelectrochemical visualisation of catalytic activity for optimisation of Direct Methanol Fuel Cells (DMFC) “, sponsored by Ministerium für Wissenschaft und Forschung des Landes Nordrhein Westfalen (223-206 003 05)

·         High sensitive electrochemical sensors by means of nanoparticle lithography for medical diagnostics and investigations of active substances (NanoBioPore)“, Project indicative NanoBioPore FKZ: 13N8607sponsored by BMBF

·         Novel Technology for Controlling Wine production and Quality (NovTech) EU – HPRN-CT-2002-00186

 

 

Education:

· Post-Doctoral stage (since May 2005) at Ruhr University Bochum, Germany in the group of Prof. Dr. Wolfgang Schuhmann – Elecktroanalytik und Sensorik

· Ph.D. 2005 Lund University, Department of Analytical Chemistry, Sweden “Functional aspects of cellobiose dehydrogenase. Applications for biosensor development”, supervisor Prof. Dr. Lo Gorton, external opponent Prof. Dr. Frieder W. Scheller

·  M.Sc. 1998 University of Bucharest, Department of Analytical Chemistry, Romania Tempus project- “Biosensors for environmental monitoring”

·  B.Sc. 1997 University of Bucharest, Department of Analytical Chemistry, Romania


 

List of Publications

 

Full papers:

11. Nan Li, Xingxing Chen, Leonard Stoica, Wei Xia, Jun Qian, Jens Aßmann, Wolfgang Schuhmann, Martin Muhler Advanced Materials (2007) accepted The catalytic synthesis of three-dimensional hierarchical carbon nanotube composites with high electrical conductivity based on electrochemical iron deposition.

10. Katarzyna Karnicka, Katrhin Echhard, Dimitrii Gushin, Leonard Stoica, Pawel J. Kulesza, Wolfgang Schuhmann Electrochem. Commun. (2007) accepted Visualisation of the local bio-electrocatalytic activity in biofuel cell cathodes by means of redox competition scanning electrochemical microscopy (RC-SECM).

9. Xingxing Chen, Nan Li, Kathrin Eckhard, Leonard Stoica, Wei Xia, Jens Assmann, Martin Muhler, Wolfgang Schuhmann Electrochem. Commun. (2007) 9, 1348–1354 Pulsed electrodeposition of Pt nanoclusters on carbon nanotubes modified carbon materials using diffusion restricting viscous electrolytes.

8. L. Stoica, T. Ruzgas, L. Gorton, 2007, submitted to BBA Electrochemical evidence of self-substrate inhibition as regulation of functions and metabolism at cellobiose dehydrogenase from Phanerochaete chrysosporium.

7. L. Stoica, T. Ruzgas, D. Haltrich, L. Gorton, Langmuir (2006) 22, 10801-10806 A comparative electrochemical investigation of cellobiose dehydrogenase from Trametes villosa and Phanerochaete chrysosporium adsorbed on graphite electrodes.

6. L. Stoica, D. Haltrich, L. Gorton, 2005 Anal. Chem. (2006) 78, 393-398 Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase.

5. L. Stoica, N. Dimcheva, D. Haltrich, T. Ruzgas, L. Gorton, Biosens. & Bioelectron. (2005) 20, 2010-2018 Electrochemical investigation of cellobiose dehydrogenase from new fungal sources on Au electrodes.

4. L. Stoica, A. Lindgren-Sjölander, T. Ruzgas, L. Gorton, Anal. Chem. 76 (2004), 4690-4696 Biosensor based on cellobiose dehydrogenase for detection of catecholamines.

3. A. Christensson, N. Dimcheva, E.E. Ferapontova, L. Gorton, T. Ruzgas, L. Stoica, S. Shleev, A.I. Yaropolov, D. Haltrich, R.N.F. Thorneley and S.D. Aust, Electroanalysis 16 (2004), 1074-1092. (Review) Direct electron transfer between lignolytic redox enzymes and electrodes.

2. C. Nistor, A. Rose, M. Farré, L. Stoica, U. Wollenberger, T. Ruzgas, D. Pfeiffer, D. Barceló, L. Gorton, J. Emnéus, Anal. Chim. Acta, 456 (2003) 3-17 In-field monitoring of cleaning efficiency in wastewater treatment plants using two phenol-sensitive biosensors.

1. A. Lindgren, L. Stoica, T. Ruzgas, A. Ciucu, L. Gorton, Analyst, 124 (1999) 527-532

Development of a cellobiose dehydrogenase modified electrode for amperometric detection of diphenols.

 

Book chapters:

3. L. Stoica, S. Neugebauer, W. Schuhmann (2007) Scanning electrochemical microscopy (SECM) as a tool in biosensor research (submitted for Advances in Biochemical Engineering/Biotechnology series; Volume: Biosensors for the 21st Century (F. Lisdat, R. Renneberg, eds.), Springer-Verlag Berlin-Heidelberg-New York

2. E. E. Ferapontova, S. Shleev, T. Ruzgas, L. Stoica, A. Christenson, J. Tkac, A. I. Yaropolov, L. Gorton (2005) in Electrochemistry of nucleic acids and proteins. Towards electrochemical sensors for genomics and proteomics (Palacek, E., Scheller, F. W. and Wang, J., eds.), Elsevier, Amsterdam

1. A. Lindgren, T. Ruzgas, L. Stoica, F. D. Munteanu, L. Gorton (2000) in Chemical and Biological Sensors for Environmental Monitoring, vol. 762 (Mulchandani, A. and Sadik, O. A., eds.), p. 113-124, ACS Symp. Ser., Washington, DC

 

Patent:

A biosensor for enzymatic detection of lactose using cellobiose dehydrogenase (CDH) immobilized on a solid support as working electrode, whereby the CDH acts via direct electron transfer pathways”, Swedish patent, April, 2005

 

 

Conferences

Oral presentation (9):

·        ISE, Edinburgh, September, 2006 – “Selective modification of nanopore electrodes as sensitive biosensing platform”

·        ECS, Denver, May, 2006 - "Biosensors Based on Different CDHs for Catecholamines and Lactose"

·        NovTech Meeting, March, 2006 – “A novel nanopore-based biosensor platform: characteristics and potential applications”

·        European Society of Electroanalytical Chemistry, Krakow, Poland, June 10-14, 2002

·        Biosensors Congress, Kyoto, Japan, May 13-15, 2002

·        Analysdagarna, Stockholm, SWEDEN, June 10-14, 2001

·        Electroanalytical Chemistry, Changchun, China, October 14-16, 2001

·        Workshop on Biomolecular Recognition Systems for Analytical Biochemistry, Luckenwalde, Germany, December 8, 2001

·        Joint Meeting Analytical Biotechnology, Luckenwalde, Germany, April 15-17, 2000

 

Poster presentation (10):

·        Biosensors Congress, Toronto, May 10-12, 2006

·        ECS, Denver, May, 2006 – “New approaches in the electrocatalytic reduction of oxygen for the biofuel cells”

·        Bioelectrochemistry, Coimbra, June 19-25, 2005

·        Electroanalysis, Thessaloniki, Grece, September 2004

·        Bioelectrochemistry & Bioenergetics, Florence, June 19-25, 2003

·        Analysdagarna, Göteborg, Sweden, June 11-13, 2003

·        Gordon Conference, Bioanalysis sensors, Ventura, CA, USA, March 10-15, 2002

·        Monitoring Molecules in Neuroscience, Dublin, Ireland, June 16-20, 2001

·        BCEIA, Changchun/Beijing, China, October 22-28, 1999

·        Analysdagarna, Uppsala, Sweden, June14-17, 1999

 

Financial rising

·      "Scandinavia-Japan Sasakawa Foundation" April, 2002                                    2.000 €

·      "KUNGL. FYSIOGRAFISKA SÄLLSKAPET I LUND"                                  800 €

·      "Svenska Kemistsamfundet" March, 2002                                                           800 €

·      ”Gordon Research Conferences”, March 2002                                                    500 $

·      “Stiftelsen Bokelunds resestipendiefond” May, 2003                         500 €

·      “FEBS, May 2004                                                                        500  £

TOTAL                     ~5.000 €


 

Other working experiences

o     Laboratory assistant (5 semesters) at analytical chemistry course: a) Atomic emission/adsorption spectroscopy; b) Graphite furnace-Atomic adsorption spectroscopy; d) Karl Fisher method for water determination; e) Introduction to electrochemistry (CV, amperometry); f) Microbore-HPLC with electrochemical detection.

o     Practical experience: biosensors development; bioelectrochemistry (characterisation of redox enzyme by amperometric and voltammetric techniques); enzyme kinetics-enzyme structure computation; protein immobilisation methods; Scanning Electrochemical Microscopy (SECM); Quartz Crystal Microbalance (QCM); Electrochemical Impedance Spectroscopy (EIS); flow injection analysis (FIA); HPLC, GC, CE, ion chromatography; circular dichroism; fluorescence, chemiluminescence, UV-spectroscopy; automated analysis; ICP-MS; ICP-AAS.

o     Research stages:

·      Ruhr University, Bochum, Germany (1 month: Nov-Dec 2002);

·      Institute of Applied Chemistry, Changchun, China (5 months: May-Jul 2000; Aug-Oct 2001)

o     Supervision

·      Stefanie Swamborn, Master student, Ruhr-Uni Bochum, 2006-2007

·      Magdalena Gebala, PhD candidate, Ruhr-Uni Bochum, 2006-2007

         “Impidimetric sensor for evaluation of non-covalent interactions of DNA”

·      Dr. Suna Timur, Ege University, Izmir, Turkey, Post-Doc, 2004

·      Dr. Nina Dimcheva, Plovdiv University, Bulgaria, Post-Doc, 2003

·      Olsson Christian, Lund University, Sweden, undergraduate student, 2001

·      Kozima Szalancz, Pecs University, Hungary, master student, 2000

o     Assistant researcher (1997-1999) National Institute of Microtechology, Bucharest, Romania

o     Salesman for BioLogic (1995) (dealer of laboratory equipment), Bucharest, Romania

PhD Courses                                            41 points

  1. KEM 514 Electrochemistry                 10 points                     January- March 1999
  2. Capillary electrophoresis                     2 points                      March 2000
  3. Information Management                     2 points                      October 2000
  4. KEM 052 Separation methods            10 points                     January- March 2001
  5. KBK 010 Biochemistry                       4 points                      April- May 2001
  6. KEM 055 Bioanalysis                         10 points                     January- March 2002
  7. Introduction to NMR                          3 points                      February-March 2003